扇贝裙边酶解制备抗氧化肽的实验研究

Abstract:) skirt was chosen for enzymatic hydrolysis, and the enzymolysis technology of scallop skirt protein were optimized to obtain antioxidant peptide.Effects of temperature, pH, hydrolysis time, enzyme quantity and solid to liquid ratio on protein hydrolyzation were studied. On the basis of single factor test, . Combined with central composite experimental design principle, response surface methodology was used to optimize the technical parameters. The results indicated that the optimal hydrolytic conditions of Response Surface Methodology were: temperature 31.20pH 9.0,hydrolysis time 1 h. The maximum scavenging rate of hydroxyl radical was 83.78%. The relative error was 0.29%,the actual experimental value was basically consistent with the predicted value of the mode1.The predicted model could reflect actual circumstances well. The results would serve as the theoretical basis for application of scallop skirt.